Identification of Bacillus anthracis by a simple protective antigen-specific mAb dot-ELISA
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چکیده
منابع مشابه
Construction of a Eukaryotic Plasmid Encoding Bacillus anthracis Protective Antigen, a Candidate for DNA Vaccine
Background: DNA immunization with plasmid DNA encoding bacterial, viral, parasitic and tumor antigens has been reported to trigger protective immunity. Objective: To evaluate the use of a DNA immunization strategy for protection against anthrax, a plasmid was constructed. Methods: The partialsequence of protective antigen of Bacillus anthracis, amino acids 175-764, as a potent immunogenic targe...
متن کاملIdentification of linear epitopes in Bacillus anthracis protective antigen bound by neutralizing antibodies.
Protective antigen (PA), the binding subunit of anthrax toxin, is the major component in the current anthrax vaccine, but the fine antigenic structure of PA is not well defined. To identify linear neutralizing epitopes of PA, 145 overlapping peptides covering the entire sequence of the protein were synthesized. Six monoclonal antibodies (mAbs) and antisera from mice specific for PA were tested ...
متن کاملContribution of immunological memory to protective immunity conferred by a Bacillus anthracis protective antigen-based vaccine.
Protective antigen (PA)-based vaccination is an effective countermeasure to anthrax infection. While neutralizing anti-PA antibody titers elicited by this vaccine serve as good correlates for protection against anthrax (S. Reuveny, M. D. White, Y. Y. Adar, Y. Kafri, Z. Altboum, Y. Gozes, D. Kobiler, A. Shafferman, and B. Velan, Infect. Immun. 69:2888-2893, 2001), no data are available on the co...
متن کاملCrystallization of the protective antigen protein of Bacillus anthracis.
The protective antigen protein, one of the three separate proteins constituting the exotoxin system of Bacillus anthracis, has been crystallized in a form suitable for structural studies. The crystal form which is most amenable to x-ray analysis is orthorhombic, space group P2(1)2(1)2(1), a = 101.1 A, b = 95.4 A, c = 87.3 A, with one protective antigen monomer/asymmetric unit. The crystals diff...
متن کاملActivity of the Bacillus anthracis 20 kDa protective antigen component
BACKGROUND Anthrax is caused by Bacillus anthracis that produce two exotoxins, lethal toxin and edema toxin. The lethal toxin is composed of the lethal factor (LF) complexed with the cell binding protective antigen (PA83, 83 kDa). Likewise, the edema factor (EF) binds to the PA83 to form the edema toxin. Once PA83 is bound to the host cell surface, a furin-like protease cleaves the full-length,...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2003
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.05027-0